GBM – Glomerular basement membrane antibody titer

Category: Rheumatology
Vasculitis & Nephrology
Test Code: 270
Disease: • Vasculitis/ Thrombosis • Nephrology
Methodology: Indirect Immunofluorescence
Substrate: Primate Kidney
Reference Range: negative <1:10
Units: Titer
Note: Positive samples at a 1:10 screening dilution are titered to an endpoint at an additional charge.
CPT Code: 86255(x2)
Schedule / Turnaround Time: Assay performed once weekly. Report availability is within one week from the time of specimen receipt.
Specimen Requirements:

Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.


Antibodies to GBM occur in glomerulonephritis and Goodpasture syndrome. Rapidly progressive glomerulonephritis (RPGN) is characterized by crescentic glomerulonephritis. RPGN may be classified into 3 types: 1) Immune complex mediated disease characterized by the presence of DNA antibodies or streptococcal antibodies 2) Glomerular basement membrane (GBM) mediated glomerulonephritis and Goodpasture syndrome 3) antineutrophil cytoplasmic antibody (ANCA) associated glomerulonephritis.