Complete CD Profile Panel includes 100, 102, 150

Category:	Gastroenterology & Hepatology
Test Code:	130
Disease:	Celiac Disease (CD)
Methodology:	Indirect Immunofluorescence, ELISA, Polymerase chain reaction (PCR) with sequence specific primers, PCR with sequence specific probe hybridization
Reference Range:	See reference ranges for individual tests.
Note:	For antibody testing: Collect 2-5 mL of blood in a red top or serum separator tube. For genetic testing: Collect 5-10mL of uncoagulated whole blood in EDTA (purple top tubes) or in ACD (yellow top tubes).
CPT Code:	86255 IFA , 83520(x3) ELISA, 83891, DNA extraction and isolation; 83900(x2), Amplification, DQA and DQB; 83896(x52) ;Hybridization with nucleic acid probes; 83912, Interpretation and reporting
Schedule / Turnaround Time:	See individual tests for scheduling and turnaround time.
Specimen Requirements:	Specimen need not be refrigerated or frozen.
Sample Stability:	Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8°C) up to five days and frozen (-20°C or lower) up to one year.
Relevance:	The detection of endomysial antibodiesaids in the diagnosis of gluten sensitive enteropathy, i.e. CD and dermatitis herpetiformis (DH). Patients with CD and DH are reported to have antibodies to endomysium, tissue transglutaminase and gliadin. Several studies have shown that endomysial antibodies have 100% specificity and sensitivity in diagnosing CD. Tissue transglutaminase antibody levels are also useful in monitoring gluten compliance of the patients. Measurement of IgG levels with deamidated gliadin is helpful for establishing diagnosis of CD in IgA deficient patients. Absence of susceptibility genes suggests the absence of disease risk with approximately 95% certainty.