Centromere antibody titer

Category: Rheumatology
Test Code: 054
Disease: Scleroderma
Methodology: Indirect Immunofluorescence
Substrate: HEp-2
Reference Range: Negative: <1:40
Units: Titer & ANA pattern reported on all positives.
Note: Positive samples at a 1:40 screening dilution are titered to 1:5120 at an additional charge.
CPT Code: 86039
Schedule / Turnaround Time: Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt.
Specimen Requirements:

Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.


Antibodies to centromere antigens are highly specific for calcinosis, Raynaud’s phenomenon, esophageal immotility, sclerodactyly, and telangiectasia, commonly referred to as CREST syndrome, a limited form of systemic sclerosis.