ANCA (anti-neutrophil cytoplasmic antibodies) titer

Category: Gastroenterology & Hepatology
Vasculitis & Nephrology
Test Code: 003
Disease: • Inflammatory Bowel Disease (IBD) • Nephrology • Vasculitis/ Thrombosis • Autoimmune Hearing Loss (SNHL)
Methodology: Indirect Immunofluorescence
Substrate: Ethanol and Formalin Fixed Human Polymorphonuclear Leukocytes
Reference Range: Negative: <1:20
Units: Titer & ANCA pattern reported on all positives.
Note: Positive samples at a 1:20 screening dilution are titered to an endpoint at an additional charge.
CPT Code: 86255
Schedule / Turnaround Time: Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt.
Specimen Requirements:

Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.


The cytoplasmic ANCA (cANCA) pattern is suggestive of Wegener’s granulomatosis and other systemic small vessel vasculitides such as microscopic polyarteritis/polyangitis, idiopathic crescentic and necrotizing glomerulonephritis. The perinuclear ANCA (pANCA) reaction patterns are observed in patients with systemic small vessel vasculitis but have also been decribed in other non-vasculitic autoimmune diseases including ulcerative colitis, Crohn’s disease and chronic hepatitis.