Actin IgA (F-Actin) ELISA
Gastroenterology & Hepatology
|Disease:||Autoimmune Liver Diseases|
|Reference Range:||Negative: <20 ; Borderline: 21.0-24.9 ; Positive ≥ 25 Units|
|Schedule / Turnaround Time:||Assay performed once every two weeks. Report availability is two weeks from the time of specimen receipt.|
Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.
Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.
The presence of IgA antibodies against F-Actin in patients having positive serologic markers for celiac disease, such as anti-endomysial, anti-transglutaminase, and/or anti-gliadin peptide IgA, correlated with more severe intestinal injury. Anti-F-Actin IgA antibodies decrease following institution of a gluten-free diet and suggest measurement of these antibodies may have a role in monitoring with gluten-free diet compliance. Positive results should be confirmed with biopsy.