Differentiation of BP from EBA on “split skin”
|Substrate:||Split Primate Skin|
|Reference Range:||Negative: <1:10|
|Note:||Positive samples at a 1:10 screening dilution are titered to an endpoint at an additional charge.|
|Schedule / Turnaround Time:||Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt.|
Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.
Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.
Epidermolysis bullosa acquisita (EBA) can mimic bullous pemphigoid (BP) clinically, histologically and immunologically. In this indirect immunofluorescence assay, EBA and BP antibodies can be distinguished by their localization in skin that is split at the lamina lucida.